MUC1 | HUABIO HA601140 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MUC1

catalog :

HA601140

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH0-47

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MUC1 antibody (HA601140) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601140) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-MUC1 antibody (HA601140) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601140) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-MUC1 antibody (HA601140) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601140) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601140

Target name :

MUC1

Species reactivity :

Human

Applications :

IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide of core peptide domain of human MUC1.

Uniprot id :

P15941>SwissProt: P15941 Human

Host :

Mouse

Clone number :

PSH0-47

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human breast carcinoma tissue, human kidney tissue, human lung carcinoma tissue, SK-Br-3 cell lysate, MCF7 cell lysate.

Molecular wt :

Predicted band size: 122 kDa

Subcellular location :

Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:1,500-1:4,000 ;WB: 1:2,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601140_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue (Negative) with Mouse anti-MUC1 antibody (HA601140) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601140) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601140_5.jpg

Pic legend5 :

Western blot analysis of MUC1 on different lysates with Mouse anti-MUC1 antibody (HA601140) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 122 kDa Observed band size: 180/250 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601140) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage