PSMD6 | HUABIO HA601127 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMD6

catalog :

HA601127

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A9E4

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSMD6 on different lysates with Mouse anti-PSMD6 antibody (HA601127) at 1/1,000 dilution. Lane 1: K562 cell lysate Lane 2: MCF-7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Hela cell lysate Lane 5: THP-1 cell lysate Lane 6: Human kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 45.5 kDa Observed band size: 40 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601127) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-PSMD6 antibody (HA601127) at 5μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601127) at 5μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601127

Target name :

PSMD6

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PSMD6 aa 1-150 / 389.

Uniprot id :

Q15008>SwissProt: Q15008 Human

Host :

Mouse

Clone number :

A9E4

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K562 cell lysate, MCF-7 cell lysate, HEK-293 cell lysate, Hela cell lysate, THP-1 cell lysate, human kidney tissue lysate, human testis tissue.

Molecular wt :

Predicted band size: 45.5 kDa

Subcellular location :

Cytosol, extracellular region, nucleoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000;IHC-P: 1:200

more info or order :

HUABIO product webpage