product summary
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company name :
HUABIO
product type :
antibody
product name :
NCAM1 / CD56
catalog :
HA601126
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
PDH0-07
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of NCAM1 / CD56 on different lysates with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 95 kDa
Observed band size: 140-250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601126) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA601126
Target name :
NCAM1 / CD56
Species reactivity :
Human
Applications :
IHC-P,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide
Uniprot id :
P13591>SwissProt: P13591 Human
Host :
Mouse
Clone number :
PDH0-07
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, Human brain tissue lysate, human appendix tissue, human brain tissue, human cerebellum tissue, human glioma tissue, human smooth muscle tissue.
Molecular wt :
Predicted band size: 95 kDa
Subcellular location :
Cell membrane, Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:400-1:3,000
;WB: 1:2,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA601126_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA601126_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human glioma tissue with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA601126_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue (human appendix) with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA601126_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue (Negative) with Mouse anti-NCAM1 / CD56 antibody (HA601126) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601126) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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