POMC | HUABIO HA601113 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

POMC

catalog :

HA601113

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PD01-40

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunofluorescence analysis of paraffin-embedded mouse pituitary tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA601113

Target name :

POMC

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human POMC aa 1-150 / 267.

Uniprot id :

P01189>SwissProt: P01189 Human;SwissProt: P01193 Mouse;SwissProt: P01194 Rat

Host :

Mouse

Clone number :

PD01-40

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Mouse pituitary tissue, rat pituitary tissue, mouse hypothalamus (arcuate nucleus) tissue.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:4,000 ;IF-Tissue: 1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601113_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded mouse hypothalamus (arcuate nucleus) tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage