FIP200 | HUABIO HA601106 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FIP200

catalog :

HA601106

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A9A9

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601106) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 µg/Lane) Lane 2: MCF-7 cell lysate (30 µg/Lane) Lane 3: 293T cell lysate (30 µg/Lane) Predicted band size: 183 kDa Observed band size: 250/150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-FIP200 antibody (HA601106) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601106) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601106) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601106

Target name :

FIP200

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human RB1CC1 aa 651-800 / 1,594.

Uniprot id :

Q8TDY2>SwissProt: Q8TDY2 Human;SwissProt: Q9ESK9 Mouse;Entrez Gene: 312927 Rat

Host :

Mouse

Clone number :

A9A9

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela cell lysate, MCF-7 cell lysate, 293T cell lysate, rat kidney tissue, human kidney tissue, Hela, MCF7, NIH/3T3.

Molecular wt :

Predicted band size: 183 kDa

Subcellular location :

Nucleus, Cytoplasm, Cytoplasm, cytosol, Lysosome.

Concentration :

1.21 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:200 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601106_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Hela cells labeling FIP200 with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601106_5.jpg

Pic legend5 :

Flow cytometric analysis of MCF7 cells labeling FIP200. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601106, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601106_6.jpg

Pic legend6 :

Flow cytometric analysis of NIH/3T3 cells labeling FIP200. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601106, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage