Kappa Light Chain | HUABIO HA601099 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Kappa Light Chain

catalog :

HA601099

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8H10

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Kappa Light Chain on human plasma lysates with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 12 kDa Observed band size: 25 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601099) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601099) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601099) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601099

Target name :

Kappa Light Chain

Species reactivity :

Human

Applications :

IHC-P,IF-Tissue,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant full protein within human IGKC.

Uniprot id :

P01834>SwissProt: P01834 Human

Host :

Mouse

Clone number :

A8H10

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human plasma lysates, human tonsil tissue, human appendix tissue.

Molecular wt :

Predicted band size: 12 kDa

Subcellular location :

Cell membrane, Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

IHC-P: 1:500 ;IF-Tissue: 1:50 ;WB: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601099_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Kappa Light Chain with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601099, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage