beta Actin | HUABIO HA601082-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Actin

catalog :

HA601082-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A2-F6-R

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601082) at 1/40,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: THP-1 cell lysate (10 µg/Lane) Lane 4: PMVEC cell lysate (10 µg/Lane) Lane 5: RH-35 cell lysate (10 µg/Lane) Lane 6: NIH/3T3 cell lysate (10 µg/Lane) Lane 7: RAW264.7 cell lysate (10 µg/Lane) Lane 8: B16F1 cell lysate (10 µg/Lane) Lane 9: MEF cell lysate (10 µg/Lane) Lane 10: Hela cell lysate (10 µg/Lane) Lane 11: F9 cell lysate (10 µg/Lane) Lane 12: PC-12 cell lysate (10 µg/Lane) Lane 13: Mouse lung cell lysate (20 µg/Lane) Lane 14: L6 cell lysate (10 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA601082-50UL

Target name :

beta Actin

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Beta-actin .

Uniprot id :

P60709>SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat

Host :

Mouse

Clone number :

A2-F6-R

Isotype :

IgG1

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, MCF-7 cell lysate, THP-1 cell lysate, PMVEC cell lysate, RH-35 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela cell lysate, F9 cell lysate, Mouse lung cell lysate, L6 cell lysate.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:80,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601082_4.jpg

Pic legend4 :

Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA601082) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601082) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage