product summary
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company name :
HUABIO
product type :
antibody
product name :
GNAS
catalog :
HA601074
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A8F12
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of GNAS on different lysates with Mouse anti-GNAS antibody (HA601074) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: NIH/3T3 cell lysate (10 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601074) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-GNAS antibody (HA601074) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA601074
Target name :
GNAS
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human GNAS aa 2-251.
Uniprot id :
P63092>SwissProt: P63092 Human;SwissProt: P84996 Human;SwissProt: Q5JWF2 Human;SwissProt: P63094 Mouse;SwissProt: Q6R0H7 Mouse;SwissProt: P63095 Rat;SwissProt: Q63803 Rat
Host :
Mouse
Clone number :
A8F12
Isotype :
IgG2a
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HepG2 cell lysate, NIH/3T3 cell lysate, human brain tissue lysate, rat pancreas tissue, human pancreas tissue, MCF-7, human breast carcinoma tissue.
Molecular wt :
Predicted band size: 46 kDa
Subcellular location :
Cell membrane
Concentration :
1.19 mg/ml
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200-1:500
;IF-Cell: 1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA601074_4.jpg
Pic legend4 :
Immunocytochemistry analysis of MCF-7 cells labeling GNAS with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) were used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA601074_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-GNAS antibody (HA601074) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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