TCP1 delta | HUABIO HA601064 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TCP1 delta

catalog :

HA601064

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8E2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TCP1 delta on different lysates with Mouse anti-TCP1 delta antibody (HA601064) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: SH-SY5Y cell lysate (10 µg/Lane) Lane 5: THP-1 cell lysate (10 µg/Lane) Lane 6: PC-3M cell lysate (10 µg/Lane) Lane 7: Jurkat cell lysate (10 µg/Lane) Lane 8: Mouse testis tissue lysate (20 µg/Lane) Lane 9: Rat spleen tissue lysate (20 µg/Lane) Lane 10: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601064) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-TCP1 delta antibody (HA601064) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601064) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601064

Target name :

TCP1 delta

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TCP1 delta aa 200-450.

Uniprot id :

P50991>SwissProt: P50991 Human;SwissProt: P80315 Mouse;SwissProt: Q7TPB1 Rat

Host :

Mouse

Clone number :

A8E2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela cell lysate, A549 cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, THP-1 cell lysate, PC-3M cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, human breast carcinoma tissue, human stomach tissue.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cytoskeleton

Concentration :

1.1 mg/ml

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:800

more info or order :

HUABIO product webpage