Human IL-6 | HUABIO HA601051 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human IL-6

catalog :

HA601051

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A6D1

reactivity :

human

application :

western blot, ELISA

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Human IL-6 on different lysates with Mouse anti-Human IL-6 antibody (HA601051) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: Human lung tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601051) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Human IL-6 on recombinant protein with Mouse anti-Human IL-6 antibody (HA601051) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601051) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA601051

Target name :

Human IL-6

Species reactivity :

Human

Applications :

WB,ELISA

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human IL-6 aa 1-212/212.

Uniprot id :

P05231>SwissProt: P05231 Human

Host :

Mouse

Clone number :

A6D1

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

SiHa cell lysate, A549 cell lysate, Hela cell lysate, human lung tissue lysate.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500 ;ELISA: 1:10,000

more info or order :

HUABIO product webpage