NAK / TBK1 | HUABIO HA601045 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NAK / TBK1

catalog :

HA601045

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8D9

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/2,000 dilution. Lane 1: K562 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Hela cell lysate Lane 4: MCF-7 cell lysate Lane 5: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si NAK / TBK1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 80 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA601045

Target name :

NAK / TBK1

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TBK1 aa 530-729/729.

Uniprot id :

Q9UHD2>SwissProt: Q9UHD2 Human;SwissProt: Q9WUN2 Mouse;SwissProt: D4A7D3 Rat

Host :

Mouse

Clone number :

A8D9

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

K562 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat stomach tissue lysate.

Molecular wt :

Predicted band size: 84 kDa

Subcellular location :

Cytoplasm

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage