product summary
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company name :
HUABIO
product type :
antibody
product name :
NAK / TBK1
catalog :
HA601044
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A8D10
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: A-172 cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Lane 9: Rat testis tissue lysate (40 µg/Lane)
Lane 10: Mouse lung tissue lysate (40 µg/Lane)
Lane 11: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601044) at 1/500 dilution.
Lane 1: A549-si NT cell lysate (20 µg/Lane)
Lane 2: A549-si NAK / TBK1 cell lysate (20 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 80 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601044) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601044
Target name :
NAK / TBK1
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human TBK1 aa 530-729/729.
Uniprot id :
Q9UHD2>SwissProt: Q9UHD2 Human;SwissProt: Q9WUN2 Mouse;SwissProt: D4A7D3 Rat
Host :
Mouse
Clone number :
A8D10
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HCT 116 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, A-172 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate.
Molecular wt :
Predicted band size: 84 kDa
Subcellular location :
Cytoplasm
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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