beta Actin | HUABIO HA601037 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Actin

catalog :

HA601037

quantity :

100μl

price :

384.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

A2-F6-R

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

image 2 :

Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

image 3 :

Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA601037) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

product information

SKU :

HA601037

Target name :

beta Actin

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

HRP-conjugated

Immunogen :

Synthetic peptide within N-terminal human Beta-actin .

Uniprot id :

P60709>SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat

Host :

Mouse

Clone number :

A2-F6-R

Isotype :

IgG1

Size :

100μl

List Price :

384.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, L6 cell lysate, MEF cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm

Concentration :

0.5 mg/mL.

Recommended dilutions :

WB: 1:20,000-1:50,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601037_4.jpg

Pic legend4 :

Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601037) at 1/10,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF-7 cell lysate Lane 3: RH-35 cell lysate Lane 4: L6 cell lysate Lane 5: MEF cell lysate Lane 6: HepG2 cell lysate Lane 7: PC-12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: RAW264.7 cell lysate Lane 10: A549 cell lysate Lane 11: PMVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601037) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

more info or order :

HUABIO product webpage