product summary
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company name :
HUABIO
product type :
antibody
product name :
CD69
catalog :
HA601013
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A8C4
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of CD69 on different lysates with Mouse anti-CD69 antibody (HA601013) at 1/500 dilution.
Lane 1: Daudi cell lysate
Lane 2: Daudi cell lysate treated with PMA for 24 hours
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601013
Target name :
CD69
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human CD69 aa 62-199/199.
Uniprot id :
Q07108>SwissProt: Q07108 Human
Host :
Mouse
Clone number :
A8C4
Isotype :
IgG2b
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
Daudi cell lysate treated with PMA for 24 hours.
Molecular wt :
Predicted band size: 23 kDa
Subcellular location :
Membrane
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:500
Advanced Validation :
Cell treatment (CT)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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