product summary
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company name :
HUABIO
product type :
antibody
product name :
CD73
catalog :
HA601011
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A6F9
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601011) at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: A375 cell lysate
Lane 3: Raji cell lysate (negative)
Lane 4: 4T1 cell lysate
Lane 5: RAW264.7 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 70 kDa
Exposure time: Lane 1: 40 seconds; Lane 2-5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601011) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA601011) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA601011
Target name :
CD73
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,FC,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human CD73 27-549.
Uniprot id :
P21589>SwissProt: P21589 Human;SwissProt: Q61503 Mouse;SwissProt: P21588 Rat
Host :
Mouse
Clone number :
A6F9
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
U-87 MG cell lysate, A375 cell lysate, 4T1 cell lysate, NCI-H441 xenograft tissue, U-87 MG xenograft tissue, human liver tissue, mouse liver tissue, rat liver tissue, U-87 MG.
Molecular wt :
Predicted band size: 63 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-P: 1:200-1:1,000
;IF-Tissue: 1:100
;FC: 1:500-1:1,000
;IF-Cell: 1:100
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA601011_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD73 antibody (HA601011) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA601011_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-CD73 antibody (HA601011) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA601011_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CD73 antibody (HA601011) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA601011_7.jpg
Pic legend7 :
Immunocytochemistry analysis of U-87 MG cells labeling CD73 with Mouse anti-CD73 antibody (HA601011) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD73 antibody (HA601011) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA601011_8.jpg
Pic legend8 :
Flow cytometric analysis of U-87 MG cells labeling CD73.
Cells were fixed and permeabilized.Then stained with the primary antibody (HA601011, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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