product summary
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company name :
HUABIO
product type :
antibody
product name :
PDE4D
catalog :
HA601001
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A7F3
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of PDE4D on different lysates with Mouse anti-PDE4D antibody (HA601001) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HT-29 cell lysate
Lane 3: C2C12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 85/91 kDa
Exposure time: Lane 1: 1 minute 2 seconds; Lane 2-3: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601001) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601001
Target name :
PDE4D
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human PDE4D aa 610-809 / 809.
Uniprot id :
Q08499>SwissProt: Q08499 Human;SwissProt: Q01063 Mouse
Host :
Mouse
Clone number :
A7F3
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, HT-29 cell lysate, C2C12 cell lysate.
Molecular wt :
Predicted band size: 91 kDa
Subcellular location :
Apical cell membrane, Cytoplasm, Cytoskeleton, Centrosome, Membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:5,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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