Immunocytochemistry analysis of 293T cells labeling Myc tag with Mouse anti-Myc tag antibody (HA600136F) at 1/1,000 dilution. 293T cells, transfected with empty control (top, negative) / Myc-tagged Histone H3.1 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myc tag antibody (HA600136F, FITC, green) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Histone H3 (ET1701-64, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.