pan Cytokeratin | HUABIO HA600135F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

pan Cytokeratin

catalog :

HA600135F

quantity :

100μl

price :

394.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PDH09-10

reactivity :

human

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of A431 cells labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600135F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600135F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600135F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA600135F

Target name :

pan Cytokeratin

Species reactivity :

Human

Applications :

IF-Cell,IF-Tissue

Conjugate :

iFluor™ 488

Immunogen :

Full length native protein corresponding to Human pan Cytokeratin.

Uniprot id :

Q01546>SwissProt: Q01546 Human;SwissProt: Q7Z794 Human

Host :

Mouse

Clone number :

PDH09-10

Isotype :

IgG1

Size :

100μl

List Price :

394.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

A431, human small intestine tissue.

Subcellular location :

Cytoplasmic

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IF-Tissue: 1:200

more info or order :

HUABIO product webpage