Beta III Tubulin | HUABIO HA600133F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Beta III Tubulin

catalog :

HA600133F

quantity :

100μl

price :

394.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8-D10

reactivity :

human, rat

application :

immunocytochemistry, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Beta III Tubulin (HA600133F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Beta III Tubulin (HA600133F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (HA600133F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (HA600133F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Flow cytometric analysis of SH-SY5Y cells labeling Beta III Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Beta III Tubulin (HA600133F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA600133F

Target name :

Beta III Tubulin

Species reactivity :

Human,Rat

Applications :

IF,ICC,FC

Conjugate :

iFluor™ 594

Immunogen :

Synthetic peptide (KLH-coupled) within human/mouse 1-50 aa.

Uniprot id :

Q13509>SwissProt: Q13509 Human;SwissProt: Q4QRB4 Rat

Host :

Mouse

Clone number :

A8-D10

Isotype :

IgG2a

Size :

100μl

List Price :

394.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Rat brain tissue, HepG2, SH-SY5Y.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm. Cytoskeleton. Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

IF: 1:100 ;ICC: 1:50 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage