beta Tubulin | HUABIO HA600110F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

beta Tubulin

catalog :

HA600110F

quantity :

100μl

price :

394.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A1-A4-R

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

image 2 :

Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (HA600110F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

product information

SKU :

HA600110F

Target name :

beta Tubulin

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IF-Tissue,FC

Conjugate :

iFluor™ 594

Immunogen :

Synthetic peptide within Human Beta tubulin aa 151-200 / 444.

Uniprot id :

P07437>SwissProt: P07437 Human;SwissProt: P99024 Mouse;SwissProt: P69897 Rat

Host :

Mouse

Clone number :

A1-A4-R

Isotype :

IgG1

Size :

100μl

List Price :

394.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa, NIH/3T3, human kidney tissue, mouse testis tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm, Cytoskeleton, Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IF-Tissue: 1:200 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA600110F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA600110F_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded rat kidney tissue labeling beta Tubulin (HA600110F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Tubulin (HA600110F, red) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA600110F_6.jpg

Pic legend6 :

Flow cytometric analysis of HeLa cells labeling beta Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA600110F_7.jpg

Pic legend7 :

Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with beta Tubulin (HA600110F, red, 1μg/mL). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage