Beta III Tubulin | HUABIO HA600106F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Beta III Tubulin

catalog :

HA600106F

quantity :

100μl

price :

394.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8-D10

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HepG2 cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (HA600106F) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (HA600106F, iFluor™ 488) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) were used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of SH-SY5Y cells labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (HA600106F) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta III Tubulin antibody (HA600106F, iFluor™ 488) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 647, HA1123) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of SH-SY5Y cells labeling Beta III Tubulin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Beta III Tubulin (HA600106F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA600106F

Target name :

Beta III Tubulin

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,FC,IF-Tissue

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide (KLH-coupled) within human Tubulin beta-3 chain aa 401-450.

Uniprot id :

Q13509>SwissProt: Q13509 Human;SwissProt: Q9ERD7 Mouse;SwissProt: Q4QRB4 Rat

Host :

Mouse

Clone number :

A8-D10

Isotype :

IgG2a

Size :

100μl

List Price :

394.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HepG2, SH-SY5Y, rat brain tissue, mouse brain tissue.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm. Cytoskeleton. Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50 ;FC: 1:500-1:1,000 ;IF-Tissue: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA600106F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (HA600106F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600106F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA600106F_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Beta III Tubulin with Mouse anti-Beta III Tubulin antibody (HA600106F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600106F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage