product summary
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company name :
HUABIO
product type :
antibody
product name :
xCT/SLC7A11
catalog :
HA600098-50UL
quantity :
50μl
price :
205.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A7C6
reactivity :
human, mouse
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of xCT/SLC7A11 on HT-29 cell lysates with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 2 minute 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600098) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of HeLa cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of A549 cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA600098-50UL
Target name :
xCT/SLC7A11
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
293 cell line overexpressing SLC7A11.
Uniprot id :
Q9UPY5>SwissProt: Q9UPY5 Human;SwissProt: Q9WTR6 Mouse
Host :
Mouse
Clone number :
A7C6
Isotype :
IgG1
Size :
50μl
List Price :
205.00 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HT-29 cell lysates, HeLa, A549, HT-29, human colon tissue, human pancreas tissue.
Molecular wt :
Predicted band size: 55 kDa
Subcellular location :
Cell membrane, Cell projection, microvillus membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:100-1:250
;IHC-P: 1:200-1:600
;FC: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA600098_4.jpg
Pic legend4 :
Immunocytochemistry analysis of HT-29 cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA600098_5.jpg
Pic legend5 :
Flow cytometric analysis of HT-29 cells labeling xCT/SLC7A11.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA600098, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA600098_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600098) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA600098_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT/SLC7A11 antibody (HA600098) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600098) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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