Histone H3 (acetyl K18) | HUABIO HA600091 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (acetyl K18)

catalog :

HA600091

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A7B6

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/2,000 dilution. Lane 1: Hela cell lysate Lane 2: Hela treated with TSA cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 14 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600091) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of TSA treated Hela cells labeling Histone H3 (acetyl K18) with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Histone H3 (acetyl K18) antibody (HA600091) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA600091

Target name :

Histone H3 (acetyl K18)

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

Uniprot id :

P68431>SwissProt: P68431 Human;SwissProt: P84243 Human;SwissProt: Q16695 Human;SwissProt: Q6NXT2 Human;SwissProt: Q71DI3 Human

Host :

Mouse

Clone number :

A7B6

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Hela treated with TSA cell lysate, Hela, TSA treated Hela.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage