Bovine Serum Albumin Antibody (HA600086) in indirect ELISA. Indirect ELISA analysis of BSA was performed by coating wells of a 96-well plate with 100 µl per well of BSA standard diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100 µl per well of a mouse BSA monoclonal antibody starting at a concentration of 0.8 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. This antibody demonstrates high specificity for BSA and little or no crossreactivity to HSA (Human serum albumin), Goat serum and Rabbit serum.

Western blot analysis of BSA on BSA protein with Mouse anti-BSA antibody (HA600086) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600086) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.