product summary
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company name :
HUABIO
product type :
antibody
product name :
AMPK alpha 2
catalog :
HA600080
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A6A12
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of AMPK alpha 2 on different lysates with Mouse anti-AMPK alpha 2 antibody (HA600080) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600080) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA600080
Target name :
AMPK alpha 2
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human AMPK alpha 2 aa 231-430.
Uniprot id :
P54646>SwissProt: P54646 Human;SwissProt: Q8BRK8 Mouse;SwissProt: Q09137 Rat
Host :
Mouse
Clone number :
A6A12
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat liver tissue lysate.
Molecular wt :
Predicted band size: 62 kDa
Subcellular location :
Nucleus, Cytoplasm.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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