product summary
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company name :
HUABIO
product type :
antibody
product name :
Mouse anti-Human IgG
catalog :
HA600076
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A5D12
application :
western blot, ELISA
more info or order :
image
image 1 :
Western blot analysis of Mouse anti-Human IgG on different lysates with Mouse anti-Mouse anti-Human IgG antibody (HA600076) at 1/1,000 dilution.
Lane 1: Recombinant human IgG protein
Lane 2: Recombinant human IgM protein
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immobilized Human IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600076) for 1 hour at 25℃.
product information
SKU :
HA600076
Target name :
Mouse anti-Human IgG
Species reactivity :
Human
Applications :
WB,ELISA
Conjugate :
Non-conjugated
Immunogen :
Full length native protein corresponding to Human IgG.
Uniprot id :
P01857>SwissProt: P01857 Human;SwissProt: P01859 Human;SwissProt: P01860 Human;SwissProt: P01861 Human
Host :
Mouse
Clone number :
A5D12
Isotype :
IgG
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
Human IgG protein.
Subcellular location :
Cell Membrane and Secreted.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;ELISA: 1:1,000-1:20,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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