CD1a | HUABIO HA600031 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD1a

catalog :

HA600031

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A4B10

reactivity :

human

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CD1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600031, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Flow cytometric analysis of HeLa (left, negative) and Jurkat (right, positive) cells labeling CD1a. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA600031, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA600031

Target name :

CD1a

Species reactivity :

Human

Applications :

IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD1a aa 50-250 (Extracellular).

Uniprot id :

P06126>SwissProt: P06126 Human

Host :

Mouse

Clone number :

A4B10

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Human skin tissue, Jurkat.

Molecular wt :

Predicted band size: 37 kDa.

Subcellular location :

Cell membrane, endosome membrane, membrane raft.

Concentration :

2 mg/ml.

Recommended dilutions :

IHC-P: 1:100-1:500 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage