product summary
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company name :
HUABIO
product type :
antibody
product name :
6X His tag
catalog :
HA600028
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A5C12
application :
western blot, ELISA
more info or order :
image
image 1 :
Western blot analysis of His-tag on C-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600028, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of His-tag on N-terminal His-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600028, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
image 3 :
Immobilized His-tagged recombinant protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.
product information
SKU :
HA600028
Target name :
6X His tag
Species reactivity :
Species independent
Applications :
WB,ELISA
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide HHHHHHGCGHHHHHH.
Uniprot id :
>
Host :
Mouse
Clone number :
A5C12
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein G affinity purified.
Product type :
Mouse monoclonal Antibody
Positive control :
C-terminal His-tagged recombinant protein lysates, N-terminal His-tagged recombinant protein lysates.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:10,000
;ELISA: Use at an assay dependent concentration.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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