NSUN4 | HUABIO HA600024 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NSUN4

catalog :

HA600024

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G3

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600024) at 1/500 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600024) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF7 cells labeling NSUN4 with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA600024

Target name :

NSUN4

Species reactivity :

Human

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human NSUN4 aa 1-384.

Uniprot id :

Q96CB9>SwissProt: Q96CB9 Human

Host :

Mouse

Clone number :

4G3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Mitochondrion

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:100

more info or order :

HUABIO product webpage