NSUN4 | HUABIO HA600023 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NSUN4

catalog :

HA600023

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4G4

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600023) at 1/500 dilution. Lane 1: MCF7 cell lysate Lane 2: HEK-293 cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: HeLa cell lysate Lane 6: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600023) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NSUN4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600023, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA600023

Target name :

NSUN4

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human NSUN4 aa 1-384.

Uniprot id :

Q96CB9>SwissProt: Q96CB9 Human;SwissProt: Q9CZ57 Mouse

Host :

Mouse

Clone number :

4G4

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, mouse testis tissue, human prostate tissue.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

Mitochondrion

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:500

more info or order :

HUABIO product webpage