Chromogranin A | HUABIO HA600016 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Chromogranin A

catalog :

HA600016

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3H1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Chromogranin A on different lysates with Mouse anti-Chromogranin A antibody (HA600016) at 1/2,000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Predicted band size: 51 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600016) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (HA600016) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Chromogranin A antibody (HA600016) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA600016

Target name :

Chromogranin A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Chromogranin A aa 358-457.

Uniprot id :

P10645>SwissProt: P10645 Human;SwissProt: P26339 Mouse;SwissProt: P10354 Rat

Host :

Mouse

Clone number :

A3H1

Isotype :

IgG

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein G affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

PC-12 cell lysates, mouse kidney tissue lysate, SH-SY5Y cell lysates, human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human medullary thyroid carcinoma tissue, human atypical carcinoid tissue.

Molecular wt :

Predicted band size: 51 kDa.

Subcellular location :

Cytoplasmic vesicle, Secreted.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:100-1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA600016_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA600016_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA600016_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue using anti-Chromogranin A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600016, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage