product summary
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company name :
HUABIO
product type :
antibody
product name :
COL4A3
catalog :
HA500591
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of COL4A3 on PC-12 cell lysates with Rabbit anti-COL4A3 antibody (HA500591) at 1/1,000 dilution.
Lysates/proteins at 15 µg/Lane.
Predicted band size: 162 kDa
Observed band size: 200 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500591) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of PC-12 cells labeling COL4A3 with Rabbit anti-COL4A3 antibody (HA500591) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL4A3 antibody (HA500591) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500591
Target name :
COL4A3
Species reactivity :
Rat
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Mouse COL4A3 aa 141-190.
Uniprot id :
>Entrez Gene: 363265 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
PC-12 cell lysates, PC-12.
Molecular wt :
Predicted band size: 162 kDa
Subcellular location :
Secreted, extracellular space, extracellular matrix, basement membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IF-Cell: 1:500
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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