product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-SMAD1 (S463/465)/ SMAD5 (S463/465)/ SMAD9 (S465/467)
catalog :
HA500583
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA500583 image 1
Western blot analysis of Phospho-SMAD1 (S463/465)/ SMAD5 (S463/465)/ SMAD9 (S465/467) on different lysates with Rabbit anti-Phospho-SMAD1 (S463/465)/ SMAD5 (S463/465)/ SMAD9 (S465/467) antibody (HA500583) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate Lane 5: HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 6: NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500583) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA500583
Target name :
Phospho-SMAD1 (S463/465)/ SMAD5 (S463/465)/ SMAD9 (S465/467)
Species reactivity :
Human,Mouse
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser463/Ser465 of Human SMAD1.
Uniprot id :
Q15797>SwissProt: Q15797 Human;SwissProt: Q99717 Human;SwissProt: O15198 Human;SwissProt: P70340 Mouse;SwissProt: P97454 Mouse;SwissProt: Q9JIW5 Mouse
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HeLa treated with 10ng/mL BMP4 for 1 hour cell lysate, NIH/3T3 treated with 10ng/mL BMP4 for 1 hour cell lysate.
Molecular wt :
Predicted band size: 52 kDa
Subcellular location :
Cytoplasm, Nucleus, Mitochondrion.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!