GM2A | HUABIO HA500560 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GM2A

catalog :

HA500560

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GM2A on different lysates with Rabbit anti-GM2A antibody (HA500560) at 1/5,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (low expression) (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (negative) (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 18 kDa Exposure time: 1 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500560) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500560

Target name :

GM2A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human GM2A aa 1-193.

Uniprot id :

P17900>SwissProt: P17900 Human;SwissProt: Q60648 Mouse;Entrez Gene: 282838 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SK-Br-3 cell lysate, HeLa cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Lysosome

Concentration :

0.2 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:200-1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500560_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GM2A antibody (HA500560) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500560_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA500560_6.jpg

Pic legend6 :

GM2A was immunoprecipitated from 0.2 mg SK-Br-3 cell lysate with HA500560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA500560 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-Br-3 cell lysate (input) Lane 2: HA500560 IP in SK-Br-3 cell lysate Lane 3: Rabbit IgG instead of HA500560 in SK-Br-3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801

more info or order :

HUABIO product webpage