L-Lactyl-alpha Tubulin (K40) | HUABIO HA500554 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

L-Lactyl-alpha Tubulin (K40)

catalog :

HA500554

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, zebrafish

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Dotblot: 1.Lac-tub primary antibody, 1:10,000 dilution (HA500554). 2. Exposure time: 60 s 3. 0.45 μm NC membrane

image 2 :

WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were transfected with HA-tagged α-tubulinWT or α-tubulinK40A plasmids. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel. Proteins were transferred to a 0.45 μm NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.

image 3 :

WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were treated with sodium lactate. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel.Proteins were transferred to a NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA500554

Target name :

L-Lactyl-alpha Tubulin (K40)

Species reactivity :

Human,Mouse,Rat,Zebrafish

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic L-Lactyl-peptide corresponding to residues surrounding Lys40 of mouse α-tubulin

Uniprot id :

P68366>SwissProt: P68366 Human;SwissProt: P68368 Mouse;SwissProt: Q5XIF6 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cytoplasm, Cytoskeleton, Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IHC-P: 1:200-1:500

more info or order :

HUABIO product webpage