product summary
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company name :
HUABIO
product type :
antibody
product name :
NSUN5
catalog :
HA500544
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: Caco-2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: A431 cell lysate
Lane 6: L-929 cell lysate
Lane 7: MEF cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Human kidney tissue lysate
Lane 10: Mouse kidney tissue lysate
Lane 11: Mouse heart tissue lysate
Lane 12: Mouse spleen tissue lysate
Lane 13: Rat kidney tissue lysate
Lane 14: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500544) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA500544
Target name :
NSUN5
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human NSUN5 aa 51-100 / 429.
Uniprot id :
Q96P11>SwissProt: Q96P11 Human;SwissProt: Q8K4F6 Mouse;Entrez Gene: 288595 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HeLa cell lysate, A549 cell lysate, Caco-2 cell lysate, K-562 cell lysate, A431 cell lysate, L-929 cell lysate, MEF cell lysate, PC-12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human skeletal muscle tissue, human placenta tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Molecular wt :
Predicted band size: 47 kDa
Subcellular location :
Nucleus, nucleolus.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-P: 1:500-1:2,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500544_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500544_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA500544_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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