product summary
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company name :
HUABIO
product type :
antibody
product name :
CrkL
catalog :
HA500536
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA500536) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: A431 cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (low expression) (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Mouse thymus tissue lysate (40 µg/Lane)
Lane 10: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 37 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of K-562 cells labeling CrkL with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Flow cytometric analysis of K-562 cells labeling CrkL.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500536, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500536
Target name :
CrkL
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,FC,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human CrkL aa 1-303 / 303.
Uniprot id :
P46109>SwissProt: P46109 Human;SwissProt: P47941 Mouse;SwissProt: Q5U2U2 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, HT-29 cell lysate, THP-1 cell lysate, A431 cell lysate, HL-60 cell lysate, C6 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, K-562, human breast cancer tissue, human ovary cancer tissue, mouse cerebellum tissue, rat cerebellum tissue.
Molecular wt :
Predicted band size: 34 kDa
Subcellular location :
Cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm.
Concentration :
1.053 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:100
;FC: 1:1,000
;IHC-P: 1:1,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500536_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CrkL antibody (HA500536) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500536) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500536_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-CrkL antibody (HA500536) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500536) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA500536_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-CrkL antibody (HA500536) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500536) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA500536_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (HA500536) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500536) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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