product summary
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company name :
HUABIO
product type :
antibody
product name :
CXCR1
catalog :
HA500526
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of CXCR1 on different lysates with Rabbit anti-CXCR1 antibody (HA500526) at 1/1,000 dilution.
Lane 1: U87-MG cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 50 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of Hela cells labeling CXCR1 with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500526
Target name :
CXCR1
Species reactivity :
Human
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within full length human CXCR1 protein.
Uniprot id :
P25024>SwissProt: P25024 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
U87-MG cell lysate, Hela cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela.
Molecular wt :
Predicted band size:
Subcellular location :
Cell membrane
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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