product summary
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company name :
HUABIO
product type :
antibody
product name :
Dicer
catalog :
HA500522
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA500522) at 1/1,000 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 218 kDa
Observed band size: 250 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of MCF-7 cells labeling Dicer with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
image 3 :
Flow cytometric analysis of C2C12 cells labeling Dicer.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500522, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500522
Target name :
Dicer
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human aa 701-1,100 / 1,922.
Uniprot id :
Q9UPY3>SwissProt: Q9UPY3 Human;SwissProt: Q8R418 Mouse
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
MCF-7 cell lysate, 293 cell lysate, Daudi cell lysate, MCF-7, C2C12.
Molecular wt :
Predicted band size: 218 kDa
Subcellular location :
Cytoplasm, perinuclear region.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000;IF-Cell: 10ug/mL;FC: 1ug/mL
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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