product summary
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company name :
HUABIO
product type :
antibody
product name :
CCPG1
catalog :
HA500520
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution.
Lane 1: HCT116 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: THP-1 cell lysate
Lane 5: SW480 cell lysate
Lane 6: HEK-293 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: C2C12 cell lysate
Lane 3: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 105 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Flow cytometric analysis of SW480 cells labeling CCPG1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500520, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500520
Target name :
CCPG1
Species reactivity :
Human,Mouse
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human CCPG1 aa 241-390 / 757.
Uniprot id :
Q9ULG6>SwissProt: Q9ULG6 Human;SwissProt: Q640L3 Mouse
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HCT116 cell lysate, A549 cell lysate, Hela cell lysate, THP-1 cell lysate, SW480 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, MEF cell lysate, SW480.
Molecular wt :
Predicted band size: 87 kDa
Subcellular location :
Cytoplasmic granule membrane.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000
;FC: 1ug/mL
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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