NOTCH3 | HUABIO HA500519 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NOTCH3

catalog :

HA500519

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NOTCH3 on different lysates with Rabbit anti-NOTCH3 antibody (HA500519) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: PANC-1 cell lysate Lane 4: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 244 kDa Observed band size: 90 kDa Exposure time: 1 minute 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500519) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of Hela cells labeling NOTCH3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500519, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA500519

Target name :

NOTCH3

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human NOTCH3 aa 2,022-2,321 / 2,321.

Uniprot id :

Q9UM47>SwissProt: Q9UM47 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, MCF-7 cell lysate, PANC-1 cell lysate, HUVEC cell lysate, Hela.

Molecular wt :

Predicted band size: 244 kDa

Subcellular location :

Cell membrane; Nucleus.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1ug/mL

more info or order :

HUABIO product webpage