WASP/Wiskott-Aldrich syndrome protein | HUABIO HA500490 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

WASP/Wiskott-Aldrich syndrome protein

catalog :

HA500490

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on rat bone marrow tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. Predicted band size: 53 kDa Observed band size: 60 kDa

image 2 :

Western blot analysis of WASP/Wiskott-Aldrich syndrome protein on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500490, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate Predicted band size: 53 kDa Observed band size: 60 kDa

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500490

Target name :

WASP/Wiskott-Aldrich syndrome protein

Species reactivity :

Human,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human WASP aa 1-200.

Uniprot id :

P42768>SwissProt: P42768 Human;Entrez Gene: 317371 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat bone marrow tissue lysates, Jurkat cell lysate, Daudi cell lysate, human spleen tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 53 kDa.

Subcellular location :

Cytoskeleton, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:600

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500490_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-WASP/Wiskott-Aldrich syndrome protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500490, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage