product summary
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company name :
HUABIO
product type :
antibody
product name :
CPN1
catalog :
HA500462
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500462, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Raji cell lysate
Lane 3: Mouse pancreas tissue lysate
Predicted band size: 52 kDa
Observed band size: 55 kDa
image 2 :
ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500462, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
product information
SKU :
HA500462
Target name :
CPN1
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human CPN1 aa 1-200 / 458.
Uniprot id :
P15169>SwissProt: P15169 Human;SwissProt: Q9JJN5 Mouse
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Hela cell lysate, Raji cell lysate, mouse pancreas tissue lysate, LOVO.
Molecular wt :
52 kDa (Predicted band size)
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500;IF-Cell: 1:50-1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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