PSMB3 | HUABIO HA500458 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMB3

catalog :

HA500458

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSMB3 on different lysates with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: K562 cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: Jurkat cell lysate (10 µg/Lane) Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500458) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. Rabbit anti-GAPDH antibody (ET1601-4) was used as loading control antibody at 1:10,000 dilution.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling PSMB3 with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Mouse anti-Beta tubulin Antibody (M1305-2) was used as Microtubule Marker at 1/200 dilution and Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded Human skin tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500458

Target name :

PSMB3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant full length protein within human PSMB3.

Uniprot id :

P49720>SwissProt: P49720 Human;SwissProt: Q9R1P1 Mouse;SwissProt: P40112 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, K562 cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa, Human skin tissue, Human breast tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 23 kDa

Subcellular location :

Cytoplasm, Nucleus, Proteasome

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:500-1:1,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500458_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded Human breast tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500458_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA500458_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA500458_7.jpg

Pic legend7 :

Flow cytometric analysis of HeLa cells labeling PSMB3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500458, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage