PPP2R5E | HUABIO HA500457 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PPP2R5E

catalog :

HA500457

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PPP2R5E on different lysates with Rabbit anti-PPP2R5E antibody (HA500457) at 1/1,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Daudi cell lysate (10 µg/Lane) Lane 3: MCF-7 cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: Human skeletal muscle tissue lysate (20 µg/Lane) Lane 6: Mouse thymus tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/48 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA500457

Target name :

PPP2R5E

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PPP2R5E aa 1-200 / 467.

Uniprot id :

Q16537>SwissProt: Q16537 Human;SwissProt: Q61151 Mouse;Entrez Gene: 299147 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, Daudi cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, Human skeletal muscle tissue lysate, Mouse thymus tissue lysate, human cervical carcinoma tissue, mouse stomach tissue, rat esophagus tissue, MCF-7.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cytoplasm

Concentration :

1.74 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:400 ;IF-Cell: 1:200 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500457_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-PPP2R5E antibody (HA500457) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500457) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA500457_5.jpg

Pic legend5 :

Immunocytochemistry analysis of MCF-7 cells labeling PPP2R5E with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP2R5E antibody (HA500457) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA500457_6.jpg

Pic legend6 :

Flow cytometric analysis of MCF-7 cells labeling PPP2R5E. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500457, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage