B3GAT1 | HUABIO HA500443 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

B3GAT1

catalog :

HA500443

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of B3GAT1 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST. for 1 hour at room temperature. The primary antibody (HA500443, 1/500) was used in 5% NFDM/TBST. at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500443, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

ICC staining of B3GAT1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500443, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA500443

Target name :

B3GAT1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human B3GAT1 aa 130-334 / 334 (Lumenal).

Uniprot id :

Q9P2W7>SwissProt: Q9P2W7 Human;SwissProt: Q9CW73 Mouse;SwissProt: O35789 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat brain tissue lysates, mouse brain tissue, SH-SY5Y.

Molecular wt :

38 kDa

Subcellular location :

Secreted, golgi apparatus membrane, endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:2,000;IHC-P:1:50-1:100;IF-Cell: 1:50-1:100;FC: 1:100-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500443_4.jpg

Pic legend4 :

Flow cytometric analysis of B3GAT1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500443, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage