PSMD13 | HUABIO HA500441 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSMD13

catalog :

HA500441

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSMD13 on different lysates with Rabbit anti-PSMD13 antibody (HA500441) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Hela cell lysate (20 µg/Lane) Lane 3: Siha cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: NIH3T3 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500441) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Siha cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of HepG2 cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA500441

Target name :

PSMD13

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PSMD13 1-160aa

Uniprot id :

Q9UNM6>SwissProt: Q9UNM6 Human;SwissProt: Q9WVJ2 Mouse;SwissProt: B0BN93 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HepG2 cell lysate, Hela cell lysate, Siha cell lysate, SH-SY5Y cell lysate, NIH3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, Siha, HepG2, Hela.

Molecular wt :

Predicted band size: 43 kDa

Subcellular location :

cytosol, extracellular region, nucleoplasm, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000;IF-Cell: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA500441_4.png

Pic legend4 :

Immunocytochemistry analysis of Hela cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA500441) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage