product summary
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company name :
HUABIO
product type :
antibody
product name :
TRPM2
catalog :
HA500437
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of TRPM2 on different lysates with Rabbit anti-TRPM2 antibody (HA500437) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 171 kDa
Observed band size: 171/120 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of TRPM2 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500437, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA500437
Target name :
TRPM2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within mouse TRPM2 aa 100-150 / 1503 (Cytoplasmic).
Uniprot id :
O94759>SwissProt: O94759 Human;SwissProt: Q91YD4 Mouse;SwissProt: Q5G856 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
THP-1 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, SH-SY5Y.
Molecular wt :
Predicted band size: 171 kDa
Subcellular location :
Lysosome, cell membrane, perikaryon, cell projection, cytoplasmic vesicle.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000
;FC: 1:100-1:500
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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