product summary
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company name :
HUABIO
product type :
antibody
product name :
Urokinase
catalog :
HA500429
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Urokinase on different lysates with Rabbit anti-Urokinase antibody (HA500429) at 1/2,000 dilution.
Lane 1: PC-3M cell lysate (10 µg/Lane)
Lane 2: MCF-7 cell lysate (10 µg/Lane)
Lane 3: LANCAP cell lysate (10 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)
Lane 5: Rat stomach tissue lysate (20 µg/Lane)
Predicted band size: 49 kDa
Observed band size: 56 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500429) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of PC-3M cells labeling Urokinase with Rabbit anti-Urokinase antibody (HA500429) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at 37℃. Cells were then incubated with Rabbit anti-Urokinase antibody (HA500429) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, Red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500429
Target name :
Urokinase
Species reactivity :
Human,Rat
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Urokinase 10- 200 aa
Uniprot id :
P00749>SwissProt: P00749 Human;SwissProt: P29598 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
PC-3M cell lysate, MCF-7 cell lysate, LANCAP cell lysate, rat kidney tissue lysate, rat stomach tissue lysate, PC-3M.
Molecular wt :
Predicted band size: 49 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1,000-2,000;IF-Cell: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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