product summary
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company name :
HUABIO
product type :
antibody
product name :
RAC1/2/3
catalog :
HA500424
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of RAC1/2/3 on different lysates with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/500 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Human brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 5 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500424) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of RAC1/2/3 on different lysates with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/500 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500424) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunocytochemistry analysis of HeLa cells labeling RAC1/2/3 with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA500424
Target name :
RAC1/2/3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human RAC3 aa 23-192 / 192.
Uniprot id :
P15153>SwissProt: P15153 Human;SwissProt: P60763 Human;SwissProt: P63000 Human;SwissProt: P60764 Mouse;SwissProt: P63001 Mouse;SwissProt: Q05144 Mouse;SwissProt: Q6RUV5 Rat;SwissProt: Q5U1Y2 Rat;SwissProt: M0R5T4 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Rat brain tissue lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, HeLa, PC-12, rat skin tissue.
Molecular wt :
Predicted band size: 21 kDa
Subcellular location :
Cytoplasm, Cytoplasm, Cell membrane.
Concentration :
1.958 mg/mL.
Recommended dilutions :
WB: 1:500
;IHC-P: 1:600
;IF-Cell: 1:100
;FC: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA500424_4.jpg
Pic legend4 :
Immunocytochemistry analysis of PC-12 cells labeling RAC1/2/3 with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA500424_5.jpg
Pic legend5 :
Flow cytometric analysis of HeLa cells labeling RAC1/2/3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500424, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA500424_6.jpg
Pic legend6 :
Flow cytometric analysis of PC-12 cells labeling RAC1/2/3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500424, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA500424_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-RAC1/2/3 antibody (HA500424) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500424) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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